Progress Towards Remote Activation of Enzymes

Research Seminar

The emerging field of remote enzyme activation, or the ability to remotely turn thermophilic enzyme function on or off, could be a valuable tool for understanding cellular processes. But with only a few preliminary reports1,2, little about the concept is understood. Through exploitation of the temperature dependence of enzymatic processes and high thermal stability of thermophilic enzymes these experiments utilize nanoparticles as ‘antennae’ that convert radiofrequency (RF) radiation into local heat, increasing activity of the enzymes without increasing the temperature of the surroundings.  RF stimulation was explored for multiple nano materials and enzymes through covalent conjugation of enzymes to nanoparticles. Nanoparticle heating properties and subsequent enzyme activation was found to be most sensitive to particle composition and size. 3

 

 

  • Suzuki, M.; Aki, A.; Mizuki, T.; Maekawa, T.; Usami, R.; Morimoto, H. Encouragement of Enzyme Reaction Utilizing Heat Generation from Ferromagnetic Particles Subjected to an AC Magnetic Field. PLOS ONE 2015, 10 (5), e0127673.
  • Kogan, M. J.; Bastus, N. G.; Amigo, R.; Grillo-Bosch, D.; Araya, E.; Turiel, A.; Labarta, A.; Giralt, E.; Puntes, V. F. Nanoparticle-Mediated Local and Remote Manipulation of Protein Aggregation. Nano Letters 2006, 6 (1), 110–115.
  • Collins, C. B.; McCoy, R. S.; Ackerson, B. J.; Collins, G. J.; Ackerson, C. J. Radiofrequency Heating Pathways for Gold Nanoparticles. Nanoscale 2014, 6 (15), 8459–8472.

Division(s): Physical

Speaker: Chris Collins

Speaker Institution: Colorado State University

Event Date: 03-02-2017

Event Time: 4:00 PM

Event Location: Chemistry A101

Mixer Time: 3:45 PM

Mixer Location: Chemistry B101E

Host: C. Ackerson