Electrochemical Aptamer-Based Techniques for Direct Analysis of Whole Blood

Literature seminar abstract

The ability to detect and quantify drugs, biomarkers, and metabolites in whole blood is important to further understand and monitor human health. Detecting these molecules in whole blood is a challenge because blood is a complex mixture difficult to analyze for specific components in a generalizable way without batch processing. Electrochemistry is an ideal detection method for direct whole blood analysis because it is rapid, inexpensive, and when coupled with biorecognition elements, it is capable of very selective and sensitive determinations. Of the many biorecognition elements, aptamers are particularly suited to whole blood analysis. Aptamers are nucleic acid-based recognition elements that can be engineered to reversibly bind to a wide range of targets and undergo large scale conformational changes upon target binding. When aptamers are labeled with redox reporters and attached to an electrode surface, binding induced conformational changes affect the rate of electron transfer which changes the electrochemical signal.  The current state of the electrochemical biosensors field will be discussed with a focus on aptamer-based sensors (E-ABs) and how recent advances in the field of E-ABs have made possible continuous monitoring of whole blood in awake ambulatory animals.

 

(1)           Arroyo-Currása, N.; Somerson, J.; Vieirad, P. A.; Ploensee, K. L.; Kippine, T. E., Plaxco, K. W., PNAS, 2017, 114 (4), 645-650

(2)           Li, H.; Curras, N. A.; Kang, D.; Ricci, F.; Plaxco, K. W., J. Am. Chem. Soc., 2016, 138 (49), 15809–15812.

(3)         Li, H.; Dauphin-Ducharme, P.; Ortega, G.; Plaxco, K. W., J. Am. Chem. Soc., 2017, 139 (32), 11207–11213.

Division(s): Analytical

Speaker: Cynthia McCord

Speaker Institution: Colorado State University

Event Date: 10-04-2017

Event Time: 4:00 PM

Event Location: Chemistry A101

Mixer Time: 3:45 PM

Mixer Location: Chemistry B101E

Host: C. Henry