Illuminating Colloidal Crystal Formation and Structure with Stimulated Emission Depletion Microscopy

Literature seminar abstract

Surpassing the barrier imposed by the diffraction of light, super-resolution microscopy distinguishes between entities closer than Abbe’s diffraction limit, ~lex/2. Stimulated Emission Depletion (STED) microscopy is one particular super-resolution technique that uses stimulated emission to shrink the focal volume of a fluorescence microscope. The technique can be tailored to observe fast dynamics, such as the formation of a colloidal monolayer 1. It can also be adapted for 3-dimensional imaging of dense crystal structures2. The talk explores the technique of STED and the insight it provides for colloidal systems. It then delves into limitations of the technique, and future directions for the field of super-resolution microscopy.


(1)           Lauterbach, M. A.; Hell, S. W. Langmuir 2010, 26, 14400-14404.


(2)           Harke, B.; Hell, S. W. Nano Lett. 2008, 8, 1309-1313.





Division(s): Physical

Speaker: Megan Dunlap

Speaker Institution: Colorado State University

Event Date: 11-16-2017

Event Time: 4:00 PM

Event Location: Chemistry A101

Mixer Time: 3:45 PM

Mixer Location: Chemistry B101E

Host: A. Van Orden