Use of 3D Microfluidic Paper-Based Analytical Devices (mPAD) for Complex Biological Assays

Literature Seminar Abstract

According to World Health Organization, infectious diseases are a leading cause of deaths globally.1 Developing cheap, fast and simple diagnostics for infectious diseases is a critical analytical challenge to enable better prevention and treatment. A conventional disease diagnostic approach is Enzyme Linked Immunosorbent Assays (ELISA). ELISA is frequently used to detect clinically relevant proteins, antibodies and small molecules. Typically, the assay is comprised of immobilized antigen to a surface that is complexed with an antibody which is then linked to an enzyme and lastly introduced to a substrate to produce measurable product. While this assay provides highly specific and sensitive quantifiable data, it is often unavailable for use in resource limited countries. Point-of-care (POC) diagnostics in the form of microfluidic paper-based analytical devices (mPADs) have been developed to circumvent the problem of insufficient availability of diagnostic tools in resource limited settings. mPADs are ideally suited to POC diagnostics due to their affordability, portability, and minimal reagent consumption.2-3 Recently, there has been a push to further automate mPADs and enhance their ease of use by storing multiple reagents on a single device for controlled sample delivery for multistep assays such as a ELISA.2-3 Sequential reagent delivery in mPADs has been performed using 3D and/or multiple layer mPADs. In this talk, I will discuss the advancement of paper-based analytical devices and the adaptation of ELISA from earliest 2D device format advancing into a 3D mPAD format, using a sliding strip for further sample control.4 The adaptation of ELISA onto 3D mPADs represents a significant step towards user friendly POC diagnostics.

 

  • Braveman, P.; Gruskin, S. Bulletin of the World Health Organization 2003, 81(7), 539–545.
  • Gong, M. M.; Sinton, D. Chemical Reviews 2017, 117, 8447-8480
  • Lim W. Y.; Goh, B. T.; Khor, S. M. Journal of Chromatography B 2017, 1060, 424-442
  • Verma, M. S.; Tsaloglou, M.-N., Sisley, T.; Christodouleas, D.; Chen, A.; Milette, J.; Whitesides, G. M. Biosensors and Bioelectronics 2018, 99, 77-84.

Division(s): Analytical

Speaker: Zarina Munshi

Speaker Institution: Colorado State University

Event Date: 11-30-2017

Event Time: 4:00 PM

Event Location: Chemistry A101

Mixer Time: 3:45 PM

Mixer Location: Chemistry B101E

Host: C. Henry