Evaluation of Sliding Strip Microfluidic Paper-Based Analytical Device (PAD) for Point-of-Care (POC) Diagnostics

Enzyme Linked Immunosorbent Assays (ELISA) are a conventional disease diagnostic approach. ELISA is frequently used to detect clinically relevant proteins, antibodies and small molecules. Typically, the assay is comprised of the antigen of interest, immobilized to a surface, that is complexed with an antibody which is then linked to an enzyme and lastly introduced to a substrate to produce measurable product. While ELISA provides highly specific and sensitive quantifiable data, it is often unavailable for use in resource limited settings. Point-of-care (POC) diagnostics in the form of microfluidic paper-based analytical devices (µPADs) have been developed to circumvent the problem of insufficient availability of diagnostic tools in resource limited settings. µPADs are ideally suited to POC diagnostics due to their affordable, fast, robust and user-friendly characteristics.1-2 Recently, there has been a push to further automate µPADs and enhance their ease of use by storing multiple reagents on a single device for controlled sample delivery for multistep assays such as a ELISA.1-2 Sequential reagent delivery in µPADs has been performed using 3D and/or multiple layer µPADs. In this talk, I will discuss and evaluate the adaptation of ELISA into a 3D µPAD format, using a sliding strip for its robustness, accessibility, sensitivity and specificity.3 The adaptation of ELISA onto 3D µPADs represents a significant step towards user friendly POC diagnostics.

 

  • Gong, M. M.; Sinton, D. Chemical Reviews 2017, 117, 8447-8480
  • Lim W. Y.; Goh, B. T.; Khor, S. M. Journal of Chromatography B 2017, 1060, 424-442
  • Verma, M. S.; Tsaloglou, M.-N., Sisley, T.; Christodouleas, D.; Chen, A.; Milette, J.; Whitesides, G. M. Biosensors and Bioelectronics 2018, 99, 77-84.

Division(s): Analytical

Speaker: Zarina Munshi

Speaker Institution: Colorado State University

Event Date: 02-01-2018

Event Time: 4:00 PM

Event Location: Chemistry A101

Mixer Time: 3:45 PM

Mixer Location: Chemistry B101E

Host: C. Henry