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SUMMARY:Characterizing Protein Assemblies in live cells using PIE-FCCS
LOCATION:Chemistry A101
TZID:America/Denver
DTSTART:20241107T160000
UID:2026-05-05-07-27-21@natsci.colostate.edu
DTSTAMP:20260505T072721
Description:About the Seminar:\n\nObserving protein interactions in live ce
 lls has remained challenging due to their heterogeneous complexities. Meth
 ods such as Fluorescence Resonance Energy Transfer (FRET) have been used t
 o track protein interactions but are limited to an energy transfer distanc
 e between molecules. Ephrin type-A Receptor 2 (EphA2) is a transmembrane 
 protein that has two functions: tumor suppression and tumor signaling de
 pending on the presence of a ligand. Previous studies that have attempted 
 to characterize these interactions using FRET have provided conflicting re
 ports about the assembly of the EphA2 assembly. The paper I will focus on 
 (Xiaojun Shi et al.\, Science 382\,1042-1050\, 2023) uses a technique call
 ed pulse interleaved excitation-fluorescence cross-correlation spectroscop
 y (PIE-FCCS) to characterize EphA2 protein interactions in cells. PIE-FCCS
  provides a multiplex readout that characterizes oligomerization\, mobilit
 y\, density\, and proximity. FCCS has been used to investigate other prote
 in-protein interactions\; the issue arises when the probe molecules underg
 o a process called crosstalk. This results in false positive signals being
  detected that are not accurate to the actual protein-protein interactions
 . PIE can be used to eliminate this crosstalk by using two different excit
 ation sources to assign photon arrival. This seminar will focus on PIE-FCC
 S as a tool to characterize the ligand-free EphA2 assembly in cells which 
 is less understood. 4:00 pm
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