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SUMMARY:Sliding-Strip microfluidic device enables ELISA on paper
LOCATION:Chemistry A101
TZID:America/Denver
DTSTART:20220216T160000
UID:2026-04-29-03-50-15@natsci.colostate.edu
DTSTAMP:20260429T035015
Description:Point-of-care testing is essential for the rapid detection of a
 nalytes that allows for management and treatment of potentially treatable 
 diseases. Current established lab techniques for quantification of such re
 quire trained scientific personnel\, external resources\, and instrumentat
 ion. One such technique is the enzyme-linked immunosorbent assay (ELISA)\,
  the gold standard for the detection and quantification of biomarkers\, wh
 ich requires multiple mixing\, incubating\, and washing steps in an ordere
 d sequence. Microfluidic paper-based analytical devices (uPADs) are valuab
 le diagnostic tools particularly in developing countries\, as they are aff
 ordable (made from paper\, lamination\, double-sided adhesive\, etc.)\, se
 nsitive\, specific\, user-friendly (no scientific background required)\, r
 apid and robust\, equipment free\, and deliverable to the end user. Howeve
 r\, uPADs are often limited to single-step biochemical assays and are unab
 le to perform complex assays such as the ELISA. Whitesides\, et al. (2018)
  presents a circumvention to the current limitations of the uPADs to perfo
 rm ELISAs by incorporating a sliding strip into the device that allows and
  makes possible to perform the required sequential steps of a traditional 
 ELISA on paper. With each component of a traditional ELISA housed within t
 he device itself and with only three end-user addition steps\, one can run
  an ELISA in a fraction of the time allowing for the rapid\, cheap\, and e
 asy detection of an analyte for point-of-care testing. 4:00 pm
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