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SUMMARY:Localization of Cryoprotectants in Plant Shoot Tips using Coherent 
 Raman Microscopy
LOCATION:Chemistry A101
TZID:America/Denver
DTSTART:20230201T160000
UID:2026-04-23-05-56-03@natsci.colostate.edu
DTSTAMP:20260423T055603
Description:About the Seminar\n\nAuthors of talk: Heidi D Kreckel\, Fionna 
 M. D. Samuels and Nancy E. Levinger\n\nGlobal plant genetic diversity is d
 ecreasing at an alarming rate due to global climate change and human popul
 ation growth. Although many conservation efforts are successful\, they are
  often at risk of pests and contamination. Further\, most ex-situ conserva
 tion methods are quite costly and require a large\, specialized staff to m
 aintain. Seed banks are considered the most cost-effective and least risky
  conservation effort but they cannot be used to conserve all plants. As a 
 result\, cryopreservation has emerged at the forefront of plant conservati
 on efforts.\n\nCryopreservation\, or the preservation of cells and tissues
  at near liquid nitrogen temperatures (-196°C)\, is considered to be extr
 emely cost-effective\, efficient and – theoretically – allows for inde
 finite storage of plant materials. Successful cryopreservation techniques 
 rely on the use of highly concentrated mixtures of cryoprotecting agents t
 o prevent freezing injury. Plant vitrification solution 2 (PVS2) – a mix
 ture of aqueous DMSO\, glycerol\, ethylene glycol and sucrose – is one o
 f the most successful mixtures used\, but it is not universally protective
 . Little is understood about the exact mechanistic role of each component 
 of the mixture in the cryoprotection of plant cells and tissues. Our goal 
 is to investigate where each component of PVS2 goes within plants cells an
 d tissues and\, subsequently\, the individual role of each cryoprotecting 
 agent within the mixture.\n\nCoherent Raman microscopy is a non-invasive m
 ethod that tracks molecules via vibrational modes. Unlike fluorescence mic
 roscopy\, which requires the use of bulky fluorophores to track molecules 
 throughout a cell\, coherent Raman microscopy relies on small vibrational 
 tags of just one or a few atoms to identify molecule location.\n\nThis tal
 k will present the significance of plant cryopreservation and demonstrate 
 applications of coherent Raman microscopy to determine the localization of
  cryoprotecting agents within peppermint shoot tips. 4:00 pm
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