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SUMMARY:Intracellular Imaging: Enhancing Contrast for Light and Cryo-Electr
 on Microscopy
LOCATION:Chemistry A101
TZID:America/Denver
DTSTART:20240911T160000
UID:2026-04-30-01-08-32@natsci.colostate.edu
DTSTAMP:20260430T010832
Description:About the Seminar:\n\nUnderstanding the cellular context of bio
 molecules at the atomic level is central for detailed chemical and mechani
 stic biological processes. Correlated light and cryo-electron microscopy 
 (cryo-CLEM) is the state-of-the-art method for correlating atomic models w
 ithin their cellular context. Cryo-electron tomography (cryo-ET) can produ
 ce 3D images of cell at 2-5 nm resolution. At this resolution\, large biom
 olecules can be identified based on their shapes\, whereas smaller biomole
 cules cannot be as reliably identified due to resolution limits. Cryo-ET p
 ractitioners have long sought out cloneable contrast agents\, analogous to
  the fluorescent proteins used widely in optical microscopy. Fluorescent p
 roteins like green fluorescent protein (GFP) are useful for localizing pro
 teins in fluorescent light microscopy because the DNA encoding a fluoresce
 nt protein can be linked to the DNA of any protein. Expression of the DNA
  results in the GFP protein covalently attached to the protein of interest
 \, where GFP fluorescence reveals the location of the protein of interest 
 within the cell. There is currently no widely applied cloneable contrast a
 gent in cryo-ET. As a result\, there is no robust cloneable contrast agent
  in cryo-CLEM due to the shortcomings of cryo-ET labels. This talk reviews
  three recent and emergent strategies for localizing proteins in cryo-ET\,
  highlighting the advances over prior state of the art and remaining limit
 ations. This talk will also discuss ongoing research in the Ackerson lab d
 eveloping cloneable contrast agents for both cryo-CLEM and cryo-ET. 4:00 p
 m
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