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SUMMARY:Peroxynitrite as a Critical Antibacterial Component of Plasma-Activ
 ated Water
LOCATION:Chemistry A101
TZID:America/Denver
DTSTART:20190301T000000
UID:2026-03-15-23-49-39@natsci.colostate.edu
DTSTAMP:20260315T234939
Description:Literature Seminar\nIn 2018\, antibiotic resistant bacterial in
 fections were the source of $2 billion (U.S.) in medical bills in the Unit
 ed States alone. With rising antibiotic resistance\, prevention of infecti
 on by chemical disinfection prior to the development of an infection is pr
 eferred over treating an infection. Production of typical chemical disinfe
 ctants\, however\, carries its own risks\, including environmental damage\
 ; thus\, endogenous cellular oxidants have become an attractive choice for
  disinfection. For example\, peroxynitrite (ONOOH)\, an endogenous cellula
 r oxidant\, is produced in cells during apoptosis and causes oxidative dam
 age to cell membranes\, proteins\, and DNA. If ONOOH could be produced in 
 a reproducible and environmentally friendly manner\, it could be a good ca
 ndidate for an effective disinfection agent. Plasma-activated water (PAW)\
 , produced by exposing water to cold atmospheric plasma\, has been shown t
 o have antibacterial properties that are believed to rely on reactive oxyg
 en and nitrogen species (RONS) in solution (e.g. nitrate\, nitrite\, and h
 ydrogen peroxide) which may ultimately form peroxynitrite. The present stu
 dy determines bactericidal effects of PAW-produced RONS in isolation and i
 n concert in order to understand the impact of peroxynitrite in PAW’s an
 tibacterial properties. A fluorescent probe for peroxynitrite using folic 
 acid selectively quantified peroxynitrite in mimicked PAW solutions and in
  PAW. Mimicked PAW solutions that form peroxynitrite prove to be almost as
  effective as PAW at killing Escherichia coli bacteria (E. coli). Another 
 probe using salicylic acid quantified the hydroxyl radicals in solution. A
 s OH is a degradant of peroxynitrite\, these experiments confirm the prese
 nce of the bactericidal species ONOOH. Studies of aged PAW demonstrate tha
 t the peroxynitrite concentration\, and concomitantly the antibacterial ef
 fect of PAW\, decreases over a short amount of time. Overall\, these resul
 ts clearly indicate the possibility of PAW being an environmentally friend
 ly disinfectant that can be produced at the point of use. 4:00 pm
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