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SUMMARY:Quantitative Examination of Insulin and Insulin-like Growth Factor-
 1 Receptor by Fluorescence Correlation Spectroscopy
LOCATION:Chemistry A101
TZID:America/Denver
DTSTART:20194101T000000
UID:2026-03-15-23-20-06@natsci.colostate.edu
DTSTAMP:20260315T232006
Description:Research Seminar Abstract\n\nThe insulin receptor (IR) and insu
 lin-like growth factor receptor (IGF1R) system is implicated in the pathog
 enesis of breast cancer. As members of the tyrosine kinase receptor family
 \, these receptors are able to dimerize in different combinations forming 
 IR-IR and IGF1R-IGF1R homodimers and IR-IGF1R heterodimers. Overexpression
  of one and/or both receptors on the cell surface represents a clinical ri
 sk factor for the disease. However\, it seems apparent that the risk is no
 t solely attributable to the absolute amounts of receptor expressed on the
  cell surface\, but also to their relative amounts and distribution betwee
 n the different dimeric forms. Despite great homology in the structures of
  and signal transduction pathways induced by these receptors\, it is theor
 ized that each species might generate differential downstream effects on c
 ell growth\, division\, and apoptosis in response to binding of hormone. T
 hus the ability to quantitate these different species and their hormone bi
 nding affinities is of paramount importance. To date\, we know of no such 
 technique able to accomplish such a feat. We propose fluorescence correlat
 ion spectroscopy\, a technique with good spatial resolution\, a large temp
 oral range\, short acquisition times\, and robust photon statistics\, as a
  method to examine membrane-localized fluorescence in situ. Herein this te
 chnique is applied to determine hormone-binding affinities and estimate re
 ceptor dimer surface densities through probe-labeled hormone-saturation ex
 periments. We also propose a new and improved method implementing our 4-de
 tector fluorescence correlation apparatus for determining receptor dimer s
 urface densities by direct labeling of receptors via transient transfectio
 n using cDNA vectors. Ultimately\, the information gained from these exper
 iments\, in tandem with endocrinological studies of hormone regulation and
  studies aiming to elucidate intracellular signaling pathways\, will shed 
 light on the role of this system in the pathophysiology of breast cancer a
 nd inform future therapeutic approaches. 4:00 pm
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