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SUMMARY:Simple Detection of Foodborne Bacteria on Paper-Based Analytical De
 vices
LOCATION:Chemistry A101
TZID:America/Denver
DTSTART:20191201T000000
UID:2026-04-28-10-25-07@natsci.colostate.edu
DTSTAMP:20260428T102507
Description:Literature Seminar\nIn 2010\, the World Health Organization rep
 orted that foodborne pathogens caused over 600 million illnesses and over 
 400\,000 deaths in that year alone.1 Because of this\, it is important to 
 be able to detect these types of pathogens to prevent outbreaks from occur
 ring. Most of the common bacterial detection methods require a centralized
  laboratory\, trained personnel\, and a significant amount of time to proc
 ess results. As an accessible alternative to traditional methods\, scienti
 sts have been looking towards paper-based analytical devices in recent yea
 rs to circumvent these problems. Because of its porous nature\, paper can 
 absorb\, dry and store chemicals deposited on it. It can induce flow via c
 apillary action without the need for instrumentation. Earlier in 2019\, th
 e Kwon group from Seoul National University of Science and Technology util
 ized these properties to design a device for the detection of two E. coli 
 strains in milk samples.2 With a single sample addition step\, fluid flows
  vertically through various layers of paper containing a detergent to lyse
  bacteria\, and oxidizing layer to adjust pH\, and a substrate layer. One 
 substrate\, X-β-gluc\, reacts with β-glucuronidase to produce a light bl
 ue color\, and the other\, S-β-gal\, reacts with β-glucuronidase making 
 a magenta color. While nearly all strains of E. coli contain both enzymes\
 , E. coli O157:H7 (a toxin producing pathogen)\, only contains β-galactos
 idase. Therefore\, in the presence of E. coli O157:H7\, the device will re
 sult in a magenta hue\; whereas\, nearly any other strain would result in 
 a blue/magenta combination. The authors of the paper were able to distingu
 ish between toxic and non-toxic strains of E. coli on the device and quant
 ify both strains with an LOD of 101 CFU/mL after a 5 hour enrichment.\n\n\
 n 	Havelaar\, A. H.\; Kirk\, M. D.\; Torgerson\, P. R.\; Gibb\, H. J.\; Ha
 ld\, T.\; Lake\, R. J.\; Praet\, N.\; Bellinger\, D. C.\; de Silva\, N. R.
 \; Gargouri\, N.\; Speybroeck\, N.\; Cawthorne\, A.\; Mathers\, C.\; Stein
 \, C.\; Angulo\, F. J.\; Devleesschauwer\, B.\; on behalf of World Health 
 Organization Foodborne Disease Burden Epidemiology Reference\, G.\, World 
 Health Organization Global Estimates and Regional Comparisons of the Burde
 n of Foodborne Disease in 2010. PLoS Med 2015\, 12 (12)\, e1001923.\n 	Kim
 \, H. J.\; Kwon\, C.\; Noh\, H.\, Paper-Based Diagnostic System Facilitati
 ng Escherichia coli Assessments by Duplex Coloration. ACS Sensors 2019\, 4
  (9)\, 2435-2441.\n\n&nbsp\; 4:00 pm
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