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SUMMARY:Bioelectrochemical Measurements with Nanoscale Pipettes.
LOCATION:Chemistry A101
TZID:America/Denver
DTSTART:20202201T000000
UID:2026-05-17-04-28-52@natsci.colostate.edu
DTSTAMP:20260517T042852
Description:About the Seminar:\nTraditional transepithelial electrical resi
 stance (TER) measurements in conjunction with molecular biology techniques
  are widely used to inform the present understanding of transport at tissu
 e interfaces. These techniques\, which include well-known Using chamber ex
 periments\, provide an excellent opportunity to study active ion transport
  and ion permeability. However\, most of these measurements represent the 
 aggregate response of thousands of transport pathways in cell membrane\, w
 hich can obfuscate measurement of individual components. We have recently 
 explored new instrumentation to perform conductance measurements at tight 
 junctions at high spatial resolution. Conductance scanning is a powerful m
 ethod in discrimination of trans- and para- cellular conductance and can p
 rovide local conductance information. By performing conductance measuremen
 ts on P-SICM\, several other advantages can be provided. With a topographi
 c image obtained with SICM\, detailed surface structures and relative posi
 tion of different cellular pathways can be obtained. Positioning of pipet 
 can be more precise and easier with the feedback system in SICM\, and cell
  damages can be avoided without pipet touching the cell membrane (virtual 
 zero). A smaller pipet (~100 nm than ~1µm) used provides a more localized
  method\, which measures conductance in a much smaller area. Also due to t
 he feedback mechanism and nanometer scale pipet\, a smaller probe surface 
 distance (~100 nm than ~3 µm) can be obtained with ease. Our most recent 
 results in conductance scanning and for increasing the chemical informatio
 n present in such studies will be discussed.\nAbout the Speaker:\nLane Bak
 er was educated at Missouri State University\, Springfield\, MO (BS Chem 1
 996) and Texas A&amp\;M University (Ph.D. 2001) working with Richard M. Cr
 ooks. He joined the group of Lloyd J. Whitman (2001) in the Surface Nanosc
 ience and Sensor Technology Section of the Naval Research Laboratory as a 
 National Research Council Postdoctoral Associate\, conducting research on 
 atomically resolved scanned probe microscopies. He then joined the group o
 f Charles R. Martin (2004) at the University of Florida as a Postdoctoral 
 Associate investigating polymeric nanopores for sensing and separations. H
 e started as an Assistant Professor at Indiana University in the Analytica
 l Chemistry Division (2006) and was promoted to Associate Professor with t
 enure in 2012. His research group is interested in three areas. (i) Transi
 ent and spatial measurement of ion concentrations with scanned probe micro
 scopies. (ii) Electrospray from nanoscale pipettes. (iii) Electrochemistry
  in small domains. 4:00 pm
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