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SUMMARY:Integrated Metabolomics for Triterpene Saponin Pathway Elucidation 
 in Medicago truncatula
LOCATION:Chemistry A101
TZID:America/Denver
DTSTART:20193201T000000
UID:2026-05-17-04-12-21@natsci.colostate.edu
DTSTAMP:20260517T041221
Description:Lloyd Sumner Ph.D.\, sponsored by Brunker Daltonics\, will be t
 he keynote speaker for the CIF Winter Symposium.\n\nAbout the Seminar\n\nT
 riterpene saponins are important bioactive plant natural products found in
  many plant families including the Leguminosae\, but much of their biosynt
 hetic pathway still remains unknown. We have used a metabolomics approach 
 integrated with gene expression profiling and genome wide associations for
  the discovery of novel pathway genes/enzymes. This approach exploited gen
 etic and biochemical diversity found within structured M. truncatula germp
 lasm core collections. UHPLC-(-)ESI-QToF-MS was used to profile saponin ac
 cumulation across a collection of 300+ M. truncatula ecotypes. The profili
 ng results identified specific ecotypes with significant differential sapo
 nin content in the root and/or aerial tissues which were selected\, and co
 rrelated gene expression profiling was performed. Correlation analyses bet
 ween gene expression and saponin accumulation revealed high correlations b
 etween saponin content with gene expression of β-amyrin synthase\, MtCYP7
 16A12\, and two cytochromes P450 genes\, MtCYP72A67 and MtCYP72A68. Cumula
 tive biochemical and molecular data support that MtCYP72A68 is a multisubs
 trate\, multifunctional oxidase and MtCYP72A67 is a 2β-hydroxylase\, both
  of which function during the early steps of triterpene-oleanate sapogenin
  biosynthesis. In a similar manner\, a novel M. truncatula sugar transfera
 se was also identified and characterized.\n\nhttps://www.research.colostat
 e.edu/cif/noco-wiski/ 4:00 pm
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