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SUMMARY:Visualizing Biology: ‘Cloneable’ Nanoparticles as Tags for Elec
 tron Microscopy
LOCATION:Chemistry A101
TZID:America/Denver
DTSTART:20191201T000000
UID:2026-05-04-05-11-49@natsci.colostate.edu
DTSTAMP:20260504T051149
Description:Research Seminar Abstract\n\nElectron microscopy (EM) has yet t
 o be readily applied to biological samples due to a lack of contrast betwe
 en the various components within a cell. Inorganic nanoparticles (NPs) and
  stains have been used for biological EM imaging however these approaches 
 generally require several levels of processing making them expensive\, ted
 ious\, and not wholly applicable.\n\nMetal reducing enzymes (MREs) could p
 rovide a biological scaffold to produce ‘Cloneable NPs’\, inorganic an
 alogs to fluorescent proteins that can be visualized with electrons and X-
 rays. Currently\, this tag is being realized by utilizing a Glutathione Re
 ductase-like Metalloid Reductase (GRLMR) isolated from an endophyte identi
 fied in the Se hyperaccumulator plant Stanleya pinnata. This GRLMR forms n
 aked Se NPs by reducing Se ions to Se(0). GRLMR can be fused to a target p
 rotein and when incubated with Se ions and the cofactor NADPH\, form and r
 etain Se NPs that can be tracked through EM and elemental analysis. GRLMR 
 provides a springboard to develop a cloneable GFP-like EM tag capable of f
 orming EM tags in situ. 4:00 pm
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