Guy Hagen, Ph.D.
BioFrontiers at University of Colorado Colorado Springs
Chemistry Research 401
About the Seminar: Several methods are now available which are able to extend the resolution of optical microscopes beyond the diffraction limit. These methods include photoactivated localization microscopy, stochastic optical reconstruction microscopy, stimulated emission depletion microscopy, and super-resolution structured illumination microscopy (SIM). In many situations, SIM is the most useful super-resolution method for imaging live cells. SIM can work with nearly all dyes and fluorescent proteins, uses efficient widefield detection, and can achieve high imaging rates. SIM does not require high illumination powers, which is a critical factor for reducing photobleaching and phototoxicity when imaging live cells. In this talk I’ll discus applications of SIM in imaging both cells and tissues, and how a combination of optical and computational methods pushes SIM in new directions.
About the Speaker: Dr. Guy Hagen attended Colorado College where he earned an undergraduate degree in Biochemistry. He then earned his PHD in analytical chemistry at Colorado State University with Dr. George Barisas. He was then a postdoctoral scholar at the Max Planck Institute for Biophysical chemistry in Göttingen, Germany. Dr. Hagen then moved to Prague, Czech Republic, where he was an assistant professor in the medical school. Today Guy is a research assistant professor at the BioFrontiers center at the University of Colorado Colorado Springs.