About the Seminar:

Quantitative analysis of biological systems and associated components is crucial in advancing our understanding of modern biological sciences. In this talk, I will share our effort in integrating analytical chemistry, bioengineering, and material chemistry for constructing assays for quantitative bioanalysis. Specifically, I would first describe a Chip-DIA strategy for streamlined single-cell proteomic analysis. The assay is composed of a custom chip and data-independent acquisition mass spectrometry (DIA-MS). Its analytical and proteomic profiling performances, as well its further extension to profile phosphoproteome from nanoscale samples (1-1000 cells) will be presented and discussed. Next, I would share efforts in constructing bio-interfaces to probe and measure integrin-mediated tension characteristics during neurite outgrowth in primary neurons, which revealed a surface viscosity-dependent neuritogenesis. These results shed light on complex biophysical mechanisms governing cell-interface interactions, providing insights to develop advanced functional bio-interfaces.

Pizza will be provided!