Julius Stuart
Speaker's Institution
Colorado State University
4:00 PM
Virtual Seminar
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Research Seminar

Barcoding technology for object tracking continues to evolve in form, complexity and security. Recently, DNA has been used as a barcode allowing the user to encode information within the nucleic sequence, increasing the material’s storage density. However, the susceptibility of DNA to environmental damage (e.g., nuclease and ultraviolet (uv) light exposure) limits the efficacy of the barcode. Porous protein crystals ‘capped’ with zinc oxide nanoparticles (ZnONPs) have the potential to house and protect DNA barcodes. While ZnONPs have been well studied, it remains less understood how the inherent properties of ZnONPs perform following immobilization around DNA barcode loaded protein crystals. Specific Aim 1 entails functionalizing ZnONPs with thiol-terminated polyethylene glycol (SH-PEG) allowing for crystal installation via surface-exposed cysteine residues. Specific aim 2 entails detecting for crystal-loaded DNA damage following incubation with nuclease and exposure to uv-light, respectively.

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