Literature Seminar

Cellular labile copper(I) as dynamic and loosely bound Cu(I) stores play an important role in cell signaling. Current fluorescent has the inconsistency issue in qualitative measurement across different samples due to the heterogeneity between cells. The new ratiometric Förster Resonance Energy Transfer (FRET) probe, FRET Copper Probe-1(FCP-1), is developed with a self-calibration nature in the hope to minimize interference from heterogeneity between cell samples. The FCP-1 is used in cellular labile copper change detection driven by oncogenes. As a side control group, a Turn-On probe, CF4, is used side by side with the FCP-1 in the same measurements. Analysis based on the data supplied by the research shows there isn’t a significant difference between the new FCP-1 probe and the Turn-On probe, CF4, in dealing with the heterogeneity between cells. Further research in other heterogeneous cellular environments like zebrafish brain tissue or lung cancer immortal cell A549 is required to provide insight into the comparison between the new FRET-based probe and the Turn-On probe.

Link to Seminar: TBA