Analyzing Oncolytic Virus Activity in Live Cells

Literature seminar abstract

Oncolytic viruses were first approved in the 1980’s as a treatment for cancer. Since then, various researchers have been engineering these viruses for increased effectiveness against tumor cells. Testing these viruses presents unique challenges, as measurements must be made in terms of the host’s response. The most common method for quantifying and identifying viruses is the plaque smear, but it requires at least four days before results can be collected, creating a need for an assay that can determine an experiment’s success in a shorter amount of time. Diallo et al created a qualitative assay in 2010 for high-throughput screening of viral metabolic activity in host cells.1 This assay uses fluorescent proteins and translated into clinical tumor samples from cancer patients. The Diallo group relied on this method again in 2016 to do a screening for compounds that could increase viral activity, this time including a similar methodology for visualizing viral activity in live mice.2

The methodology in both these papers will be examined along with future directions.

 

(1) Diallo et al. Mol. Ther. 2010. 18, 1123-1129.

(2) Dornan et al. Sci. Rep. 2016. 6, 1-23.

 

 

Division(s): Analytical

Speaker: Cameron Van Cleave

Speaker Institution: Colorado State University

Event Date: 02-14-2018

Event Time: 4:00 PM

Event Location: Chemistry A101

Mixer Time: 3:45 PM

Mixer Location: Chemistry B101E

Host: D. Crans